Confocal Raman Microscopy

This second edition provides a cutting-edge overview of physical, technical and scientific aspects related to the widely used analytical method of confocal Raman microscopy.

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Author: Jan Toporski

Publisher: Springer

ISBN: 9783319753805

Category: Science

Page: 596

View: 666

This second edition provides a cutting-edge overview of physical, technical and scientific aspects related to the widely used analytical method of confocal Raman microscopy. The book includes expanded background information and adds insights into how confocal Raman microscopy, especially 3D Raman imaging, can be integrated with other methods to produce a variety of correlative microscopy combinations. The benefits are then demonstrated and supported by numerous examples from the fields of materials science, 2D materials, the life sciences, pharmaceutical research and development, as well as the geosciences.

Label Free Analysis of Drug Delivery Systems and Cellular Interaction Studies Using Confocal Raman Microscopy

In pharmaceutical development many questions still remain unsolved despite the availability of many analytical techniques. Consequently, the need of novel analytical approaches is not yet satisfied.

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Author: Birthe Kann

Publisher: Cuvillier Verlag

ISBN: 9783736983212

Category: Medical

Page: 151

View: 982

In pharmaceutical development many questions still remain unsolved despite the availability of many analytical techniques. Consequently, the need of novel analytical approaches is not yet satisfied. In this thesis, confocal Raman microscopy is utilized to fill the scientific gap. In fact, the benefit of this non-destructive, label-free visualization technique for profound analysis in complex pharmaceutical applications is successfully demonstrated. The impact of drying on drug distribution is proven by localizing the drug in wet-extruded pellets with Raman imaging. Additionally to this finding, the correlation between drug distribution and release is successfully elucidated. For the first time, confocal Raman microscopy is combined with optical profilometry. Thus, the limitations of the confocal microscope are overcome and all-encompassing component visualization in complex drug delivery systems exhibiting challenging structured surfaces is realized. During development of a lipid-based drug permeation model, the successive formation of the permeation barrier during coating is finally described using Raman analysis. Investigations benefit tremendously from a combination of chemical imaging in lateral and vertical planes to depict barrier integrity and stability. Finally, human cells as well as the uptake of different nanoparticles are analyzed label-free in aqueous environment, utilizing linear and coherent Raman techniques.

Raman Microscopy

One of the first books devoted entirely to the subject of Raman microscopy, Raman Microscopy addresses issues of great interest to engineers working in Raman-microscope development and researchers concerned with areas ofapplication for this ...

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Author: George Turrell

Publisher: Academic Press

ISBN: 0080540252

Category: Science

Page: 463

View: 417

One of the first books devoted entirely to the subject of Raman microscopy, Raman Microscopy addresses issues of great interest to engineers working in Raman-microscope development and researchers concerned with areas ofapplication for this science. The book is written by several world recognized experts, who summarize the Raman effect before discussing the hardware and software involved in todays instruments. This format provides an excellent introduction to this up-and-coming discipline. All important applications, including those in materials science and earth science are covered in depth. Includes extensive description of the instrumentation, the Raman microspectrograph, the treatment of data, and micro-Raman imaging Examines the use of Raman microscopy in diverse applications, including some of the hyphenated methods Summarizes the Raman effect Discusses new uses for this technology

Modern Raman Microscopy

This book presents the techniques of confocal Raman microscopy and imaging for researchers and engineers from a variety of disciplines. It highlights the key aspects of this technique in order to effectively apply it in practice.

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Author: Alexander Rzhevskii

Publisher: Cambridge Scholars Publishing

ISBN: 9781527570634

Category: Science

Page: 392

View: 988

Raman microscopy is now well-established as one of the most powerful and versatile techniques for a diverse range of applications in both research and analytical laboratories. Its unique advantage is its ability to noninvasively characterize chemically complex and spatially inhomogeneous samples with a sub-micron spatial resolution. Modern confocal Raman scanning microscopy, which allows one to obtain two- and three-dimensional spectrochemical images of samples in various states and forms, has become a method of choice for a wide range of applications including the study of biological cells, tissues, and microorganisms, characterization of pharmaceutical drugs and formulations, forensic evidence, minerals and gems, carbon nanomaterials, semiconductors, composite polymers, and more. This book presents the techniques of confocal Raman microscopy and imaging for researchers and engineers from a variety of disciplines. It highlights the key aspects of this technique in order to effectively apply it in practice. It will appeal to a wide circle of readers who are interested in, or are already, using the methods of confocal Raman microscopy and imaging in their work, and will also be beneficial for novice Raman microscopy users.

Pharmaceutical Applications of Raman Spectroscopy

Pharmaceutical applications of Raman spectroscopy have developed similarly and this book will focus on those applications.

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Author: Slobodan Sasic

Publisher: John Wiley & Sons

ISBN: 0470225874

Category: Science

Page: 280

View: 565

Raman spectroscopy has advanced in recent years with increasing use both in industry and academia. This is due largely to steady improvements in instrumentation, decreasing cost, and the availability of chemometrics to assist in the analysis of data. Pharmaceutical applications of Raman spectroscopy have developed similarly and this book will focus on those applications. Carefully organized with an emphasis on industry issues, Pharmaceutical Applications of Raman Spectroscopy, provides the basic theory of Raman effect and instrumentation, and then addresses a wide range of pharmaceutical applications. Current applications that are routinely used as well as those with promising potential are covered. Applications cover a broad range from discovery to manufacturing in the pharmaceutical industry and include identifying polymorphs, monitoring real-time processes, imaging solid dosage formulations, imaging active pharmaceutical ingredients in cells, and diagnostics.

Infrared and Raman Spectroscopy

This book fully integrates the use of both IR and Raman spectroscopy as spectral interpretation tools, enabling the user to utilize the strength of both techniques while also recognizing their weaknesses.

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Author: Peter Larkin

Publisher:

ISBN: 0128041625

Category: SCIENCE

Page: 260

View: 872

Infrared and Raman Spectroscopy, Principles and Spectral Interpretation, Second Edition provides a solid introduction to vibrational spectroscopy with an emphasis on developing critical interpretation skills. This book fully integrates the use of both IR and Raman spectroscopy as spectral interpretation tools, enabling the user to utilize the strength of both techniques while also recognizing their weaknesses. This second edition more than doubles the amount of interpreted IR and Raman spectra standards and spectral unknowns. The chapter on characteristic group frequencies is expanded to include increased discussions of sulphur and phosphorus organics, aromatic and heteroaromatics as well as inorganic compounds. New topics include a discussion of crystal lattice vibrations (low frequency/THz), confocal Raman microscopy, spatial resolution in IR and Raman microscopy, as well as criteria for selecting Raman excitation wavelengths. These additions accommodate the growing use of vibrational spectroscopy for process analytical monitoring, nanomaterial investigations, and structural and identity determinations to an increasing user base in both industry and academia. Integrates discussion of IR and Raman spectra Pairs generalized IR and Raman spectra of functional groups with tables and text Includes over 150 fully interpreted, high quality IR and Raman reference spectra Contains fifty-four unknown IR and Raman spectra, with a corresponding answer key

Infrared and Raman Spectroscopy of Polymers

Confocal Raman microscopy was used for the investigation of variations in crosslinking with depth in thicknesses of up to 0.5 mm in UV-cured coatings. Variations of curing with depth in pigment formulations, in thicknesses of up to 30 ...

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Author: J. L. Koenig

Publisher: iSmithers Rapra Publishing

ISBN: 9781859572849

Category: Science

Page: 152

View: 320

Vibrational spectroscopy is advantageous as an analytical tool for polymers and comprises two complementary techniques: infrared (IR) and Raman spectroscopy. This report is an absorbing overview of how these methods can be employed to provide information about complex polymeric macromolecules with respect to composition, structure, conformation and intermolecular interactions. The review is supported by several hundred abstracts selected from the Polymer Library giving useful references for further reading.

Coherent Raman Scattering Microscopy

The First Book on CRS MicroscopyCompared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation.

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Author: Ji-Xin Cheng

Publisher: CRC Press

ISBN: 9781439867662

Category: Science

Page: 610

View: 120

The First Book on CRS Microscopy Compared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Scattering Microscopy explains how CRS can be used to obtain a point-by-point chemical map of live cells and tissues. In color throughout, the book starts by establishing the foundation of CRS microscopy. It discusses the principles of nonlinear optical spectroscopy, particularly coherent Raman spectroscopy, and presents the theories of contrast mechanisms pertinent to CRS microscopy. The text then provides important technical aspects of CRS microscopy, including microscope construction, detection schemes, and data analyses. It concludes with a survey of applications that demonstrate how CRS microscopy has become a valuable tool in biomedicine. Due to its label-free, noninvasive examinations of living cells and organisms, CRS microscopy has opened up exciting prospects in biology and medicine—from the mapping of 3D distributions of small drug molecules to identifying tumors in tissues. An in-depth exploration of the theories, technology, and applications, this book shows how CRS microscopy has impacted human health and will deepen our understanding of life processes in the future.

Handbook of Raman Spectroscopy

2 C. Spatial Characterization Through Confocal Raman Microscopy 1. PET Everall [ 66 ] used confocal Raman microscopy to determine P200 on each side of a drawn film . He found that one side had a higher P200 than the other and that the ...

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Author: Ian R. Lewis

Publisher: CRC Press

ISBN: 1420029258

Category: Science

Page: 1072

View: 727

This work covers principles of Raman theory, analysis, instrumentation, and measurement, specifying up-to-the-minute benefits of Raman spectroscopy in a variety of industrial and academic fields, and how to cultivate growth in new disciplines. It contains case studies that illustrate current techniques in data extraction and analysis, as well as over 500 drawings and photographs that clarify and reinforce critical text material. The authors discuss Raman spectra of gases; Raman spectroscopy applied to crystals, applications to gemology, in vivo Raman spectroscopy, applications in forensic science, and collectivity of vibrational modes, among many other topics.