Experimental Protocols in Biotechnology

This manual provides insights into the theory and practical aspects of several biotechnological and biochemical techniques for plants, protozoa, nematodes, insects and fishes, as well as human samples.

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Author: Neelima Gupta

Publisher: Humana

ISBN: 1071606093

Category: Science

Page: 246

View: 799

This manual provides insights into the theory and practical aspects of several biotechnological and biochemical techniques for plants, protozoa, nematodes, insects and fishes, as well as human samples. The book also covers bioinformatics tools. The manual is an inclusive compilation, explaining techniques for microbial cultures, their diagnostics, DNA barcoding, microscopic techniques, blood analysis, parasite diagnostics through copro-antigens, enzyme analysis with enzyme kinetics, gene expression analysis, in-vivo protein visualization in live animals, geno-toxicity assays, quantification of micro RNAs and LncRNAs in tissue sections, the use of droplet PCR, and in-silico analysis. It provides step-by-step descriptions and details of each methodology, together with the final outcomes and inferences, in a simple and lucid manner, easily reproducible even for beginners. The broad range of techniques covered makes this volume extremely useful in understanding the principles of biotechniques, and simple applications for practical courses.

Molecular Biology and Biotechnology

The book aims to provide ample support to both students and faculty while conducting practical lessons. Four sections are covered in this book—Genomics, Proteomics, Quantitative Biochemistry, and Bioinformatics.

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Author: M. P. Bansal

Publisher: The Energy and Resources Institute (TERI)

ISBN: 9788179933794

Category: Science

Page: 392

View: 535

Molecular Biology and Biotechnology: basic experimental protocols is a compilation of methods and techniques commonly used in biomedical and biotechnological studies. The book aims to provide ample support to both students and faculty while conducting practical lessons. Four sections are covered in this book—Genomics, Proteomics, Quantitative Biochemistry, and Bioinformatics. A concise introductory note accompanies each protocol/method described for better comprehension. Every topic discussed is supported by actual methods and their expected results, and is accompanied by relevant questions.

Plant Tissue Culture

A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.

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Author: B. N. Sathyanarayana

Publisher: I. K. International Pvt Ltd

ISBN: 9788189866112

Category: Botany, Experimental

Page: 316

View: 471

Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.

Analytical Techniques in Biochemistry and Molecular Biology

This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

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Author: Rajan Katoch

Publisher: Springer Science & Business Media

ISBN: 1441997857

Category: Science

Page: 441

View: 787

Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Experimental Process Biotechnology Protocols

This laboratory manual containing protocols is prepared on the basis of my personal learning exposures at Jadavpur University, Kolkata, experiences and supervision of laboratory work in microbial and enzyme engineering and technology ...

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Author: Satya Narayan Mukhopadhyay

Publisher:

ISBN: 8130905981

Category: Biotechnology

Page: 214

View: 114

Experimental Process Biotechnology is one of the basic requirements for Biochemical Engineering, Bioprocess Engineering and Biotechnology. Theoretical basis of this course encampusing analytical bio-chemistry, microbiology, cell biology,biochemical and chemical engineering sciences, general and applied molecular biology, plant and animal cell culture engineering etc. is now well founded. This laboratory manual containing protocols is prepared on the basis of my personal learning exposures at Jadavpur University, Kolkata, experiences and supervision of laboratory work in microbial and enzyme engineering and technology conducted at Biochemical Engineering Research Centre, Deptt of Biochemical Engineering & Biotechnology, IIT Delhi, Fermentation technology laboratory, Osaka University, Japan and other places.

Experimental Biotechnology

The idea behind this practical manual was thus to provide theoretical basis of the practical study items to be undertaken in the laboratory in a lucid manner.

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Author: Sunita Dutta

Publisher: Nipa

ISBN: 9380235720

Category: Science

Page: 282

View: 785

The book is subdivided into seven sections this encompass: general procedures, like methods of pipetting, solution preparation, buffers and principles of common analytical instruments essential for laboratory biotechnology experiments. The book also includes working with nucleic acid, bacteria, enzymes, proteins; cloning experiments and a few protocols on plant biotechnology. Emphasis have been given on DNA/RNA isolation from various sources, use of restriction enzymes, ligation techniques, cloning protocols, screening of transformed cells, various electrophoresis techniques, PCR protocol, etc. The appendices in the last part are included to provide information important to he study of the above-mentioned practical as a whole. The book will be useful to students belonging to Biotechnology, agriculture and allied fields. The idea behind this practical manual was thus to provide theoretical basis of the practical study items to be undertaken in the laboratory in a lucid manner.

Handbook of Hydrocarbon and Lipid Microbiology

This handbook provides a comprehensive overview of microbial interactions with the major forms of hydrocarbons, oils, and lipids in or entering the biosphere.

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Author: Terry McGenity

Publisher: Springer

ISBN: 3540775889

Category: Science

Page: 4699

View: 373

This handbook provides a comprehensive overview of microbial interactions with the major forms of hydrocarbons, oils, and lipids in or entering the biosphere. It is the definitive resource on the physiological mechanisms and adaptive strategies characteristic of the microbial lifestyle that plays out at hydrophobic material: aqueous liquid interfaces.

Laboratory Protocols in Applied Life Sciences

This book covers a wide spectrum of areas in the interdisciplinary fields of life sciences, pharmacy, medical and paramedical sciences, and biotechnology.

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Author: Prakash Singh Bisen

Publisher: CRC Press

ISBN: 9781466553149

Category: Medical

Page: 1826

View: 232

As applied life science progresses, becoming fully integrated into the biological, chemical, and engineering sciences, there is a growing need for expanding life sciences research techniques. Anticipating the demands of various life science disciplines, Laboratory Protocols in Applied Life Sciences explores this development. This book covers a wide spectrum of areas in the interdisciplinary fields of life sciences, pharmacy, medical and paramedical sciences, and biotechnology. It examines the principles, concepts, and every aspect of applicable techniques in these areas. Covering elementary concepts to advanced research techniques, the text analyzes data through experimentation and explains the theory behind each exercise. It presents each experiment with an introduction to the topic, concise objectives, and a list of necessary materials and reagents, and introduces step-by-step, readily feasible laboratory protocols. Focusing on the chemical characteristics of enzymes, metabolic processes, product and raw materials, and on the basic mechanisms and analytical techniques involved in life science technological transformations, this text provides information on the biological characteristics of living cells of different origin and the development of new life forms by genetic engineering techniques. It also examines product development using biological systems, including pharmaceutical, food, and beverage industries. Laboratory Protocols in Applied Life Sciences presents a nonmathematical account of the underlying principles of a variety of experimental techniques in disciplines, including: Biotechnology Analytical biochemistry Clinical biochemistry Biophysics Molecular biology Genetic engineering Bioprocess technology Industrial processes Animal Plant Microbial biology Computational biology Biosensors Each chapter is self-contained and written in a style that helps students progress from basic to advanced techniques, and eventually design and execute their own experiments in a given field of biology.

Protocols for Macroalgae Research

This book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research.

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Author: Bénédicte Charrier

Publisher: CRC Press

ISBN: 9781351132947

Category: Medical

Page: 496

View: 893

This book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research. A major focus will be on bioenergy related aspects of this emerging technology. These protocols will be written in a clear and concise manner using simple language permitting even nonspecialist to adequately understand the significance of this research. It will also contain all necesssary notes and guidelines for successful execution of these experiments.

NanoBiotechnology Protocols

Illuminating and cross-disciplinary, NanoBiotechnology Protocols enables novice and experienced researchers alike to quickly come up to speed with both nanomaterials preparation and the use of nanomaterials in biological and medicinal ...

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Author: Sandra J Rosenthal

Publisher: Humana Press

ISBN: 1627038094

Category: Science

Page: 230

View: 544

Hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The author's emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology, and cutting-edge techniques in the emerging nanobiotachnology arena.