Methods in Actinobacteriology

This volume details techniques on the study of Isolation, characterization, and exploration of actinobacteria in industrial, food, agricultural, and environmental microbiology.


Author: Dhanasekaran Dharumadurai

Publisher: Humana

ISBN: 1071617273

Category: Science

Page: 747

View: 813

This volume details techniques on the study of Isolation, characterization, and exploration of actinobacteria in industrial, food, agricultural, and environmental microbiology. Chapters cover a wide range of basic and advanced techniques associated with research on isolation, characterization and identification of actinobacteria in soil, sediment, estuarine, water, Saltpan, Mangroves, plants, lichens, sea weeds, sea grass, animals-crab, snail, shrimp. Authoritative and cutting-edge, Methods in Actinobacteriology aims to be a useful practical guide to researches to help further their study in this field.

Actinobacteria Diversity and Biotechnological Applications

Many researchers and various companies have targeted the isolation of novel actinobacteria from different plants that have a high ethnobotanical value, in addition to using different treatment methods for surface sterilization for the ...


Author: Bhim Pratap Singh

Publisher: Elsevier

ISBN: 9780444639950

Category: Science

Page: 362

View: 746

Actinobacteria: Diversity and Biotechnological Applications: New and Future Developments in Microbial Biotechnology and Bioengineering, a volume in the series New and Future Developments in Microbial Biotechnology and Bioengineering series, offers the latest on the biotechnology of Kingdom actinobacteria, covering unique niches like their endosphere, rhizospheric soil and contaminated sites, etc. The book also covers the bioactive secondary metabolites obtained from actinobacteria and describes the application of microorganism (Actinobacteria) in plant growth promotion and in environmental cleanup. Finally, the book describes the biocontrol aspects of actinobacteria and how they can control fungal phytopathogens and the production of secondary metabolites. Includes an overview of all types of actinobacteria, source and enzymatic activity Lists various bioengineering methods for the production of these enzymes Reviews numerous industrial applications of actinobacteria, i.e., crop improvement, removal of heavy metals, etc. Offers unique coverage of the application of actinobacteria in bioremediation processes Explores the plant growth promoting potential of endophytic actinobacteria Describes biosynthetic potential genes associated with actinobacterial genome


There are two main manners for enzymes modification to adapt their functions rational redesign and combinatorial methods. 3.1. Rational redesign This strategy is based on using direct mutation to hit amino acid substitutions, ...


Author: Dharumadurai Dhanasekaran

Publisher: BoD – Books on Demand

ISBN: 9789535122487

Category: Science

Page: 400

View: 597

This book presents an introductory overview of Actinobacteria with three main divisions: taxonomic principles, bioprospecting, and agriculture and industrial utility, which covers isolation, cultivation methods, and identification of Actinobacteria and production and biotechnological potential of antibacterial compounds and enzymes from Actinobacteria. Moreover, this book also provides a comprehensive account on plant growth-promoting (PGP) and pollutant degrading ability of Actinobacteria and the exploitation of Actinobacteria as ecofriendly nanofactories for biosynthesis of nanoparticles, such as gold and silver. This book will be beneficial for the graduate students, teachers, researchers, biotechnologists, and other professionals, who are interested to fortify and expand their knowledge about Actinobacteria in the field of Microbiology, Biotechnology, Biomedical Science, Plant Science, Agriculture, Plant pathology, Environmental Science, etc.


Moreover, the genomic era has given many recent techniques to further strengthen the identification of several NP BGCs from the genomes of actinomycetes (Tao et al. 2018). Although different approaches like RNA polymerase engineering ...


Author: Loganathan Karthik

Publisher: Springer Nature

ISBN: 9789811658358

Category: Science

Page: 276

View: 922

This book summarizes the basics of actinobacteria, from microbiology to synthetic biology. It focuses on diversity, NRPS, sesquiterpenes, lantipeptide, bioinformatics apparatuses, cloning, CRISPR, reverse engineering, FDA supported medications, and marine actinobacteria. It also covers the latest trends in drug discovery from actinobacteria, and introduces several recently developed bioinformatics and synthetic biology tools to explore new antibiotics from actinobacteria. Many natural products such as polyketides, isoprenoids, phenazines, peptides, indolocarbarbazoles, sterols, and others have been isolated and characterized from actinobacteria. Some products are synthesized by the non-ribosomal peptide synthetases (NRPSs), polyketide synthases (PKSs), or other functional genes. Although genome sequencing has uncovered the differing qualities of these chemicals, recognizing new items and their biosynthetic pathways is still under examination. Cryptic metabolic pathways have been explored using molecular techniques or culture-dependent approaches. In recent years, researchers’ primary interest is to identify the specific conditions or agents that wake the cryptic antibiotics. Several bioinformatics and synthetic biology tools were developed to explore new antibiotics from actinobacteria. The book comprises 14 chapters with different aspects of application and utilization of actinomycetes from the microbiology; systems biology, pharmacology of natural products, bioinformatics, actinomycete and its diversity, CRISPR, artificial Intelligence, synthetic biology, metabolic engineering, expressional studies, and biosynthetic gene clusters. The book delivers useful information on actinomyces to researchers, novices in genome designing, specialists, clinicians, policymakers, and professionals.


Among the techniques actually used in the removal of heavy metals include physicochemical methods (soil washing, excavation, ion exchange columns, treatment with alkaline solutions, flocculating agents, precipitation, ion exchange, ...


Author: Maria Julia Amoroso

Publisher: CRC Press

ISBN: 9781466578746

Category: Science

Page: 296

View: 591

This book describes isolated actinobacteria from different environments, and how these can be used to bioremediate heavy metals and pesticides in contaminated sites. It also describes how free-living actinobacteria acquire the capability to produce nodules in plants and how this factor could be important for accelerating the degradation of pesticides in soils or slurries. Some chapters show how actinobacteria can be used to produce industrial enzymes and metabolites under different physicochemical conditions for use in the food industry. This book will interest professionals involved with waste management, environmental protection, and pollution abatement.


Among the first cultivation techniques developed were dilution cultures combined with fiow cytometry, in which marine bacteria were diluted and then cultivated in seawater based-media (Button et al. 1993). The most probable number (MPN) ...



Publisher: Springer Nature

ISBN: 9789811633539

Category: Actinobacteria

Page: 330

View: 368

Through this book, the readers will learn about the different aspects of Actinobacteria- beginning with its ecology and occurrence, to the ways of its adaptation to harsh climates, and finally to its practical applications. The book also presents methods of identifying and characterizing this diverse group of bacteria through advanced techniques like MALDI-TOF, 16S rRNA analysis, etc. Different chapters describe the various biotechnological applications of Actinobacteria, including bioremediation, secondary metabolite production, and in producing antibiotics, anti-cancer therapeutics. It also provides insights into the applications in agriculture and forestry by inhibiting plant pathogenic bacteria's growth. .

Beneficial Microbes in Agro Ecology

Some other methods, such as the membrane filter method (use of 0.22 to 0.45 μm pore size cellulose ester membrane ... The actinobacteria should be isolated using culturable or unculturable methods to explore the exact diversity and ...


Author: N. Amaresan

Publisher: Academic Press

ISBN: 9780128235584

Category: Business & Economics

Page: 932

View: 971

Beneficial Microbes in Agro-Ecology: Bacteria and Fungi is a complete resource on the agriculturally important beneficial microflora used in agricultural production technologies. Included are 30 different bacterial genera relevant in the sustainability, mechanisms, and beneficial natural processes that enhance soil fertility and plant growth. The second part of the book discusses 23 fungal genera used in agriculture for the management of plant diseases and plant growth promotion. Covering a wide range of bacteria and fungi on biocontrol and plant growth promoting properties, the book will help researchers, academics and advanced students in agro-ecology, plant microbiology, pathology, entomology, and nematology. Presents a comprehensive collection of agriculturally important bacteria and fungi Provides foundational knowledge of each core organism utilized in agro-ecology Identifies the genera of agriculturally important microorganisms

Plant Growth Promoting Actinobacteria

15.6.1 Biological Synthesis of Nanoparticles Using Actinomycetes Many methods are available for the synthesis of metal nanoparticles. Basically, there are two approaches used to synthesize nanoparticles, namely, top-down and bottom-up ...


Author: Gopalakrishnan Subramaniam

Publisher: Springer

ISBN: 9789811007071

Category: Technology & Engineering

Page: 298

View: 262

Global yields of legumes have been relatively stagnant for the last five decades, despite the adoption of conventional and molecular breeding approaches. The use of plant growth-promoting (PGP) bacteria for improving agricultural production, soil and plant health has become one of the most attractive strategies for developing sustainable agriculture. Actinomycetes are bacteria that play an important role in PGP and plant protection, produce secondary metabolites of commercial interest, and their use is well documented in wheat, rice, beans, chickpeas and peas. In order to promote legumes, the general assembly of the UN recently declared 2016 the “International Year of Pulses.” In view of this development, this book illustrates how PGP actinomycetes can improve grain yield and soil fertility, improve control of insect pests and phytopathogens, and enhance host-plant resistance. It also addresses special topics of current interest, e.g. the role of PGP actinomycetes in the biofortification of legume seeds and bioremediation of heavy metals.

Advances in Actinobacteria Research and Application 2012 Edition

These methods together with arabionose/melezitose fermentation analysis clearly differentiated only the collection strains into B. longum ssp. longum and B. longum ssp. infantis at the subspecies level. On the other hand, ARDRA analysis ...



Publisher: ScholarlyEditions

ISBN: 9781481610247

Category: Medical

Page: 70

View: 153

Advances in Actinobacteria Research and Application / 2012 Edition is a ScholarlyBrief™ that delivers timely, authoritative, comprehensive, and specialized information about Actinobacteria in a concise format. The editors have built Advances in Actinobacteria Research and Application / 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Actinobacteria in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Advances in Actinobacteria Research and Application / 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at

Actinobacteria and Myxobacteria

Further, recent cultivation-independent analysis using 16S rRNA gene-based methods revealed that actinobacteria can be specifically enriched in plant roots, and are more abundant in diseased plants than in healthy plants, ...


Author: Joachim Wink

Publisher: MDPI

ISBN: 9783039435296

Category: Science

Page: 270

View: 792

Bacterial infections cause millions of deaths globally, particularly in children and the elderly, and four of the 10 leading causes of death are infectious diseases in low- and middle-income countries. The continuous use of antibiotics has resulted in multi-resistant bacterial strains all over the world, such as Community-associated Methicillin-resistant Staphylococcus aureus (MRSA), extended-spectrum β-lactamases (ESBLs), and, as expected, hospitals have become breeding grounds for human-associated microorganisms, especially in critical care units.